Chopped and diced: Dicer1 deletion generates myeloid dysplasia.
نویسنده
چکیده
Gene targeting studies revealed that Dicer1 is required for murine embryogenesis.In this issue of Blood, Alemdehy and colleagues examine deletion of Dicer1 in myeloid progenitor cells using a conditional Cebpa-Cre allele. They show that deletion of Dicer1 is required for viability and that Dicer1 regulates steps of neutrophil maturation.
منابع مشابه
Dicer1 deletion in myeloid-committed progenitors causes neutrophil dysplasia and blocks macrophage/dendritic cell development in mice.
MicroRNAs (miRNAs) have the potential to regulate cellular differentiation programs; however, miRNA deficiency in primary hematopoietic stem cells (HSCs) results in HSC depletion in mice, leaving the question of whether miRNAs play a role in early-lineage decisions un-answered. To address this issue, we deleted Dicer1, which encodes an essential RNase III enzyme for miRNA biogenesis, in murine ...
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MicroRNAs (miRNAs) belong to an abundant class of highly conserved small (22nt) non-coding RNAs. MiRNA profiling studies indicate that their expression is highly cell type-dependent. DICER1 is an essential RNase III endoribonuclease for miRNA processing. Hematopoietic cell type- and developmental stage-specific Dicer1 deletion models show that miRNAs are essential regulators of cellular surviva...
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nucleus by the Drosha RNase into premiRNAs (comprising 60 to 70 nucleotides). After transport into the cytoplasm, the premiRNAs are further processed by an RNase enzyme termed Dicer. miRNAs (and experimentally designed siRNAs) are generated consisting of 22 nucleotide RNA duplexes with two-nucleotide 3 overhangs. Dicer is part of the RNA-induced silencing complex that functions downstream of mi...
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Microarray studies have shown that individual synthetic small interfering RNAs (siRNAs) can have substantial off-target effects. Pools of siRNAs, produced by incubation of dsRNAs with recombinant Dicer or RNase III, can also be used to silence genes. Here we show that diced siRNA pools are highly complex, containing hundreds of different individual siRNAs. This high complexity could either comp...
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Dicer, a member of the RNase III family, is the key enzyme required for the biogenesis of microRNAs and small interfering RNAs. Recent evidence indicates that DICER1 expression levels vary among different solid tumors and decreased or increased DICER1 expression has been associated with aggressive cancers. In this study, we assessed DICER1 expression levels in acute myeloid leukemia (AML) and i...
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ورودعنوان ژورنال:
- Blood
دوره 119 20 شماره
صفحات -
تاریخ انتشار 2012